6F6D

The catalytic domain of KDM6B in complex with H3(17-33)K18IA21M peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	77
Detector technology	PIXEL
Collection date	2017-07-05
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.072
Spacegroup name	P 41 21 2
Unit cell lengths	68.480, 68.480, 230.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	57.500 - 1.818
R-factor	0.166994950638
Rwork	0.165
R-free	0.21359
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5oy3
RMSD bond length	0.010
RMSD bond angle	1.033
Data scaling software	autoPROC (1.1.7)
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	65.633	1.850
High resolution limit [Å]	1.818	1.818
Rmerge	0.125
Rmeas	0.131
Rpim	0.037
Number of reflections	49999	2490
<I/σ(I)>	12.3	2.1
Completeness [%]	99.2	100
Redundancy	12.3	13.2
CC(1/2)	0.998	0.730

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	277	0.1M TRIS pH 8.0, 15%(v/v) Tert-butanol,