5OY3

The structural basis of the histone demethylase KDM6B histone 3 lysine 27 specificity

Summary for 5OY3

• Entry DOI: 10.2210/pdb5oy3/pdb
• Descriptor: Lysine-specific demethylase 6B, Histone 3 peptide H3(17-33)A21M, FE (III) ION, ... (8 entities in total)
• Functional Keywords: epigenetics, histone demethylase, substrate complex, oxidoreductase
• Biological source: Homo sapiens (Human); More
• Total number of polymer chains: 2
• Total formula weight: 60577.30

Authors

Jones, S.E.,Olsen, L.,Gajhede, M. (deposition date: 2017-09-07, release date: 2017-09-20, Last modification date: 2024-01-17)

Primary citation

Jones, S.E.,Olsen, L.,Gajhede, M.
Structural Basis of Histone Demethylase KDM6B Histone 3 Lysine 27 Specificity.
Biochemistry, 57:585-592, 2018

PubMed Abstract: KDM subfamily 6 enzymes KDM6A and KDM6B specifically catalyze demethylation of di- and trimethylated lysine on histone 3 lysine 27 (H3K27me3/2) and play an important role in repression of developmental genes. Despite identical amino acid sequence in the immediate surroundings of H3K9me3/2 (ARKS), the enzymes do not catalyze demethylation of this general marker of repression. To address this question for KDM6B, we used computational methods to identify H3(17-33)-derived peptides with improved binding affinity that would allow co-crystallization with the catalytic core of human KDM6B (ccKDM6B). A total of five peptides were identified, and their IC values were determined in a matrix-assisted laser desorption ionization time-of-flight-based assay. Despite none of the peptides showing affinity significantly higher than that of the H3(17-33) peptide, it was possible to co-crystallize ccKDM6B with a H3(17-33)A21M peptide. This structure reveals the interactions between the KDM6B zinc binding domain and the H3(17-23) region. Although KDM6A and KDM6B differ in primary sequence, particularly in the H3L20 binding pocket of the zinc binding domains, where two histidines in KDM6A have been replaced by a glutamate and a tyrosine, they bind H3(17-23) in a very similar fashion. This structure shows that KDM6B, in analogy with KDM6A, also uses the zinc binding domain to achieve H3K27me3/me2 specificity. The histidine to glutamine substitution at amino acid position 1564 in the KDM6B zinc binding domain can further explain why KDM6B binds substrates with an affinity higher than that of KDM6A.

PubMed: 29220567
DOI: 10.1021/acs.biochem.7b01152

Experimental method

X-RAY DIFFRACTION (2.136 Å)