6F4T
Human JMJD5 (W414C) in complex with Mn(II), NOG and RCCD1 (139-143) (complex-5)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-07-10 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 49.137, 65.169, 78.446 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.234 - 1.220 |
R-factor | 0.1419 |
Rwork | 0.141 |
R-free | 0.15440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6f4r |
RMSD bond length | 0.018 |
RMSD bond angle | 1.511 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.130 | 1.290 |
High resolution limit [Å] | 1.220 | 1.220 |
Rmerge | 0.049 | 1.036 |
Rpim | 0.023 | 0.481 |
Number of reflections | 75316 | 10919 |
<I/σ(I)> | 13.3 | 1.8 |
Completeness [%] | 99.6 | 99.9 |
Redundancy | 5.6 | |
CC(1/2) | 0.999 | 0.623 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 0.1M Bis-Tris pH 6.5, 17.1 % PEG3350, 0.002 M MnCl2, 300 nl sitting drops (sample:well, 1:1 ratio) |