6ERH

Complex of XLF and heterodimer Ku bound to DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-03
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.98015
Spacegroup name	P 1 21 1
Unit cell lengths	111.847, 118.925, 128.219
Unit cell angles	90.00, 93.11, 90.00

Refinement procedure

Resolution	49.380 - 2.800
R-factor	0.226
Rwork	0.225
R-free	0.25200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1jey
RMSD bond length	0.007
RMSD bond angle	0.950
Data reduction software	XDS
Phasing software	MOLREP
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.380	3.160
High resolution limit [Å]	2.800	2.800
Rmerge	0.164	1.350
Rmeas	0.182	1.470
Rpim	0.094	0.760
Number of reflections	41644
<I/σ(I)>	9.1	1.5
Completeness [%]	50.4	8.5
Redundancy	7.1	6.9
CC(1/2)	0.997	0.600

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	18% PEG 3350, 150 mM sodium sulfate, and 100 mM Bis-Tris-Propane