6ERG

Complex of XLF and heterodimer Ku bound to DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-11
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.98009
Spacegroup name	P 1 21 1
Unit cell lengths	111.710, 114.260, 127.170
Unit cell angles	90.00, 93.14, 90.00

Refinement procedure

Resolution	48.760 - 2.900
R-factor	0.22
Rwork	0.218
R-free	0.24400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1jey
RMSD bond length	0.007
RMSD bond angle	0.940
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.760	3.090
High resolution limit [Å]	2.900	2.900
Rmerge	0.171	2.480
Rmeas	0.182	2.560
Rpim	0.068	0.940
Number of reflections	51644
<I/σ(I)>	9.7	1
Completeness [%]	72.9	21
Redundancy	14	14.2
CC(1/2)	0.996	0.510

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	18% PEG 3350, 150 mM Na sulfate , 100 mM Bis-Tris-Propane