6EAQ
Glycosylated FCGR3B / CD16b in complex with afucosylated IgG1 Fc
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2017-03-10 |
Detector | DECTRIS PILATUS3 X 1M |
Wavelength(s) | 1.00 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 123.629, 49.388, 139.415 |
Unit cell angles | 90.00, 103.04, 90.00 |
Refinement procedure
Resolution | 45.695 - 2.220 |
R-factor | 0.2153 |
Rwork | 0.213 |
R-free | 0.24450 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5vu0 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.073 |
Data reduction software | HKL-3000 (Rv704w) |
Data scaling software | HKL-3000 (Rv704w) |
Phasing software | PHASER (1.10.1-2155) |
Refinement software | PHENIX (1.10.1-2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.695 | 2.299 |
High resolution limit [Å] | 2.220 | 2.220 |
Rmerge | 0.177 | 2.410 |
Number of reflections | 40439 | 3536 |
<I/σ(I)> | 7.01 | |
Completeness [%] | 97.0 | |
Redundancy | 3.5 | |
CC(1/2) | 0.974 | 0.755 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | pH 6.0 50 mM MES, 8% PEG3350 and 60 mM NaCl |