6E67

Structure of beta2 adrenergic receptor fused to a Gs peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	80
Detector technology	PIXEL
Collection date	2016-05-11
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.000
Spacegroup name	P 21 21 2
Unit cell lengths	83.010, 377.440, 45.590
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.980 - 3.700
R-factor	0.2794
Rwork	0.276
R-free	0.30720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4lde
RMSD bond length	0.003
RMSD bond angle	0.433
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.600	3.800
High resolution limit [Å]	3.700	3.700
Number of reflections	16163	1257
<I/σ(I)>	4.15	1.05
Completeness [%]	99.0	98.8
Redundancy	7.5	4.5
CC(1/2)	0.980	0.510

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	7	293	100 mM Bis-Tris, pH 7.0, 75-125 mM ammonium acetate, 50-75 mM potassium fluoride, 38-42% PEG400, 6% ethylene glycol, 1.5% 1,2-propaneiol