6DXW

Human N-acylethanolamine-hydrolyzing acid amidase (NAAA) precursor (C126A)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-09-17
Detector	RAYONIX MX-300
Wavelength(s)	0.98
Spacegroup name	P 1 21 1
Unit cell lengths	87.909, 140.011, 90.123
Unit cell angles	90.00, 115.20, 90.00

Refinement procedure

Resolution	38.257 - 2.300
R-factor	0.1679
Rwork	0.167
R-free	0.19330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5u7z
RMSD bond length	0.005
RMSD bond angle	0.851
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.300	2.300
Number of reflections	87300
<I/σ(I)>	18.5
Completeness [%]	100.0
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	1 M LiCl, 0.1 M MES pH 6 and 10 % PEG 6000