6DO1
Structure of nanobody-stabilized angiotensin II type 1 receptor bound to S1I8
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2018-03-24 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.033 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 135.682, 227.796, 41.500 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.600 | 3.080 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 1.457 | |
Rmeas | 0.410 | 1.697 |
Number of reflections | 29111 | 4662 |
<I/σ(I)> | 2.1 | 0.9 |
Completeness [%] | 98.5 | 99.3 |
Redundancy | 3.6 | 3.6 |
CC(1/2) | 0.962 | 0.562 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 8 | 293.15 | Protein was reconstituted with a 10:1 (w:w) mix of monoolein and cholesterol. Crystals were grown in cubic phase sandwich plates with using 100 mM Tris pH 8.0, 15-25 mM MgCl2, and 28-29% PEG 300 |