6DO1

Structure of nanobody-stabilized angiotensin II type 1 receptor bound to S1I8

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Type-1 angiotensin II receptor,Soluble cytochrome b562 BRIL fusion protein	polymer	425	48514.9	2	UniProt (P30556) UniProt (P0ABE7) Pfam (PF00001)	Homo sapiens (Human)	AT1AR,AT1BR,Angiotensin II type-1 receptor,AT1,Cytochrome b-562,AT1AR,AT1BR,Angiotensin II type-1 receptor,AT1
2	C, D, E, F (C, D, E, F)	Nanobody Nb.AT110i1	polymer	126	13712.0	4		synthetic construct
3	G, H (G, H)	Angiotensin-like peptide S1I8	polymer	8	970.2	2		synthetic construct
4	I, O (A, B)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	2	Chemie (NAG)
5	J, K, L, P, Q (A, B)	CHLORIDE ION	non-polymer		35.5	5	Chemie (CL) PubChem (312)
6	M, N, R, S, T (A, B)	(2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate	non-polymer		356.5	5	Chemie (OLC) PubChem (25021708) PubChem (54294270) PubChem (46840179) PubChem (33022) PubChem (54294271) PubChem (5283468) PubChem (5364833) PubChem (11451146) PubChem (12178130)
7	AA, U, V, W, X... (H, A, B, C, D...)	water	water		18.0	76	Chemie (HOH)

Sequence modifications

A, B: 2 - 226 (UniProt: P30556)

PDB	External Database	Details
Asp -6	-	expression tag
Tyr -5	-	expression tag
Lys -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Asp -1	-	expression tag
Asp 0	-	expression tag
Lys 1	-	expression tag

A, B: 227 - 325 (UniProt: P0ABE7)

PDB	External Database	Details
Trp	Met 29	engineered mutation

A, B: 326 - 418 (UniProt: P30556)

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	153818.1
Non-Polymers*	Number of molecules	12
	Total formula weight	2402.4
All*	Total formula weight	156220.5

*Water molecules are not included.