6DJW
Crystal Structure of pParkin (REP and RING2 deleted)-pUb-UbcH7 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-01-28 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 134.606, 134.606, 86.193 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.280 - 3.801 |
| R-factor | 0.2647 |
| Rwork | 0.263 |
| R-free | 0.29680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6djx |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.793 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.31) |
| Phasing software | PHASER (2.8.0) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.280 | 48.280 | 4.250 |
| High resolution limit [Å] | 3.800 | 8.500 | 3.800 |
| Rmerge | 0.077 | 0.036 | 1.093 |
| Rmeas | 0.083 | 0.039 | 1.173 |
| Rpim | 0.030 | 0.015 | 0.421 |
| Total number of observations | 67854 | ||
| Number of reflections | 9162 | 874 | 2560 |
| <I/σ(I)> | 14.3 | ||
| Completeness [%] | 99.9 | 99.3 | 100 |
| Redundancy | 7.4 | 6.4 | 7.5 |
| CC(1/2) | 0.999 | 0.999 | 0.695 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 0.1 M Tris-HCl, pH 8.0, 15% w/v PEG2000 MME, 0.1 M potasssium chloride |
