6DJW

Crystal Structure of pParkin (REP and RING2 deleted)-pUb-UbcH7 complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	RBR-type E3 ubiquitin transferase,RBR-type E3 ubiquitin transferase	polymer	349	39413.9	1	UniProt (A0A034W4L8) Pfam (PF00240) Pfam (PF17976) Pfam (PF17978)	Bactrocera dorsalis (Oriental fruit fly)	Parkin
2	B (B)	Ubiquitin	polymer	74	8542.7	1	UniProt (P62992) Pfam (PF00240)	Bos taurus (Bovine)
3	C (C)	Ubiquitin-conjugating enzyme E2 L3	polymer	159	18327.1	1	UniProt (P68036) Pfam (PF00179)	Homo sapiens (Human)	E2 ubiquitin-conjugating enzyme L3, L-UBC, UbcH7, Ubiquitin carrier protein L3, Ubiquitin-conjugating enzyme E2-F1, Ubiquitin-protein ligase L3
4	D, E, F, G, H... (A)	ZINC ION	non-polymer		65.4	6	Chemie (ZN)

Sequence modifications

A: 29 - 160 (UniProt: A0A034W4L8)

PDB	External Database	Details
Gly 20	-	expression tag
Gly 21	-	expression tag
Glu 22	-	expression tag
Asn 23	-	expression tag
Leu 24	-	expression tag
Tyr 25	-	expression tag
Leu 26	-	expression tag
Gly 27	-	expression tag
Gly 28	-	expression tag

A: 161 - 412 (UniProt: A0A034W4L8)
C: 1 - 154 (UniProt: P68036)

PDB	External Database	Details
Gly -4	-	expression tag
Pro -3	-	expression tag
Leu -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Lys 86	Cys 86	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	66283.7
Non-Polymers*	Number of molecules	6
	Total formula weight	392.5
All*	Total formula weight	66676.1

*Water molecules are not included.