6D8X
PPAR gamma LBD complexed with the agonist GW1929
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-11-02 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 61.934, 61.934, 167.920 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.305 - 1.900 |
| R-factor | 0.208 |
| Rwork | 0.205 |
| R-free | 0.24460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5tto |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.596 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.305 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmeas | 0.650 | |
| Number of reflections | 26487 | 2520 |
| <I/σ(I)> | 13.5 | 2.3 |
| Completeness [%] | 99.2 | 97.3 |
| Redundancy | 8 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 200mM tri-Lithium Citrate 20% (w/v) PEG 3350 |
