6BVA

Ubiquitin Variant (UbV.Fl10.1) bound to a human Skp1-Fbl10 fragment complex.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	93.15
Detector technology	PIXEL
Collection date	2016-11-21
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97910
Spacegroup name	P 21 21 21
Unit cell lengths	65.360, 81.793, 128.685
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.460 - 2.660
R-factor	0.218
Rwork	0.216
R-free	0.26300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ubq 5ibk 5jh5
RMSD bond length	0.006
RMSD bond angle	0.881
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.760
High resolution limit [Å]	2.660	2.660
Number of reflections	20716
<I/σ(I)>	16.5	2.2
Completeness [%]	99.8	99.9
Redundancy	5.3	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.1 M Hepes pH 7.5, 0.2 M calcium chloride, 18% (w/v) PEG 6000