6AKO

Crystal Structure of FOXC2 DBD Bound to DBE2 DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	Cu FINE FOCUS
Temperature [K]	85
Detector technology	CCD
Collection date	2016-03-06
Detector	RIGAKU
Wavelength(s)	1.5870
Spacegroup name	P 21 21 21
Unit cell lengths	42.540, 72.924, 99.047
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.970 - 2.396
R-factor	0.23
Rwork	0.228
R-free	0.25030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5x07
RMSD bond length	0.003
RMSD bond angle	0.611
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.970	2.482
High resolution limit [Å]	2.396	2.396
Rmerge	0.041	0.302
Number of reflections	11726	780
<I/σ(I)>	34.65
Completeness [%]	92.0
Redundancy	12.1
CC(1/2)	1.000	0.979

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	50 mM Bis-Tris propane (pH 6.68), 14% PEG4K, 200 mM NaCl, 10 mM MgCl2, 1 mM TCEP