5ZV2
FGFR-1 in complex with ligand lenvatinib
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-11-20 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 210.214, 57.464, 65.955 |
Unit cell angles | 90.00, 106.75, 90.00 |
Refinement procedure
Resolution | 100.650 - 2.860 |
R-factor | 0.21475 |
Rwork | 0.213 |
R-free | 0.26931 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4uwy |
RMSD bond length | 0.008 |
RMSD bond angle | 1.232 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.650 | 3.110 |
High resolution limit [Å] | 2.860 | 2.860 |
Number of reflections | 17573 | |
<I/σ(I)> | 21.87 | |
Completeness [%] | 99.1 | 98 |
Redundancy | 3.7 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 277 | 17.70 % PEG-5000-MME, 0.20 M (NH4)2SO4, 0.10 M Tris pH 7.00, Cryo 25% Ethylenglycole in reservoir solution. |