5ZMC
Structural Basis for Reactivation of -146C>T Mutant TERT Promoter by cooperative binding of p52 and ETS1/2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE MASSIF-1 |
Synchrotron site | ESRF |
Beamline | MASSIF-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-01-27 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 1.00 |
Spacegroup name | P 41 2 2 |
Unit cell lengths | 71.414, 71.414, 262.520 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 71.410 | 3.220 |
High resolution limit [Å] | 2.990 | 2.990 |
Rmerge | 0.060 | 0.800 |
Number of reflections | 15992 | 2514 |
<I/σ(I)> | 20.3 | |
Completeness [%] | 99.9 | 100 |
Redundancy | 6.7 | 7.2 |
CC(1/2) | 0.990 | 0.820 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 290 | 0.1 M HEPES pH 7.0 and 2.0 M Ammonium sulphate |