5ZKP

Crystal structure of the human platelet-activating factor receptor in complex with SR 27417

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-05-26
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0000
Spacegroup name	P 32 2 1
Unit cell lengths	67.050, 67.050, 280.250
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.810
R-factor	0.224
Rwork	0.222
R-free	0.25900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4xnv 1f4p
RMSD bond length	0.010
RMSD bond angle	1.080
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.950
High resolution limit [Å]	2.800	2.800
Rpim	0.087	0.622
Number of reflections	18902
<I/σ(I)>	16.9
Completeness [%]	99.4
Redundancy	7.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE		293	HEPES, PEG 400, NaSCN, Na citrate