5ZKJ

Human EXOG-H140A in complex with RNA/DNA hybrid duplex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	80
Detector technology	CCD
Collection date	2017-09-20
Detector	RAYONIX MX300HE
Wavelength(s)	1.000
Spacegroup name	H 3
Unit cell lengths	167.576, 167.576, 105.438
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.434 - 2.798
R-factor	0.1831
Rwork	0.181
R-free	0.22690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5t4i
RMSD bond length	0.003
RMSD bond angle	0.487
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.880
High resolution limit [Å]	2.798	2.798
Rmerge	0.049	0.468
Rmeas	0.066	0.640
Rpim	0.044	0.435
Number of reflections	25994	2202
<I/σ(I)>	13.6	1.747
Completeness [%]	95.8	97.7
Redundancy	1.9	1.8
CC(1/2)	0.934	0.706

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	277	0.1 M MES pH 6.0, 22% PEG 400