5ZK5
Stapled-peptides tailored against initiation of translation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-06-27 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.983998 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 37.480, 65.090, 76.390 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.250 |
R-factor | 0.20176 |
Rwork | 0.198 |
R-free | 0.26839 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4tpw |
RMSD bond length | 0.009 |
RMSD bond angle | 1.748 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.680 |
High resolution limit [Å] | 1.590 | 1.590 |
Number of reflections | 9319 | 1457 |
<I/σ(I)> | 16.11 | 3.13 |
Completeness [%] | 99.4 | |
Redundancy | 8.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | 30%(w/v) PEG6000, 0.1M Na-Hepes pH 6.5 |