5ZK5
Stapled-peptides tailored against initiation of translation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-27 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.983998 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.480, 65.090, 76.390 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.250 |
| R-factor | 0.20176 |
| Rwork | 0.198 |
| R-free | 0.26839 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4tpw |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.748 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.680 |
| High resolution limit [Å] | 1.590 | 1.590 |
| Number of reflections | 9319 | 1457 |
| <I/σ(I)> | 16.11 | 3.13 |
| Completeness [%] | 99.4 | |
| Redundancy | 8.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | 30%(w/v) PEG6000, 0.1M Na-Hepes pH 6.5 |
