5ZK1
Crystal Structure of the CRTC2(SeMet)-CREB-CRE complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-03-18 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97918 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 50.271, 242.878, 40.548 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.932 - 3.050 |
R-factor | 0.232 |
Rwork | 0.228 |
R-free | 0.26860 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1dh3 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER (2.8.2) |
Refinement software | PHENIX (1.11.1-2575_1692) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.100 |
High resolution limit [Å] | 3.050 | 8.270 | 3.050 |
Rmerge | 0.137 | 0.064 | 1.513 |
Rmeas | 0.149 | 0.070 | 1.660 |
Rpim | 0.056 | 0.029 | 0.657 |
Number of reflections | 5064 | 301 | 229 |
<I/σ(I)> | 9.9 | ||
Completeness [%] | 96.6 | 99.3 | 89.1 |
Redundancy | 6.9 | 6.1 | 5 |
CC(1/2) | 0.998 | 0.635 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | 25% w/v polyethylene glycol 3,350, 0.1 M hepes (pH 7.5), 0.2 M lithium sulfate, 0.3 M sodium chloride |