5ZG1
Crystal structure of the GluA2o LBD in complex with glutamate and Compound-2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.3 |
Synchrotron site | ALS |
Beamline | 5.0.3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-07-10 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.976486 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 47.105, 64.623, 90.291 |
Unit cell angles | 90.00, 90.01, 90.00 |
Refinement procedure
Resolution | 40.000 - 1.320 |
R-factor | 0.16169 |
Rwork | 0.161 |
R-free | 0.18354 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ftj |
RMSD bond length | 0.011 |
RMSD bond angle | 1.467 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.340 |
High resolution limit [Å] | 1.320 | 1.320 |
Number of reflections | 122835 | 4506 |
<I/σ(I)> | 17.9 | 2.2 |
Completeness [%] | 96.6 | 70.7 |
Redundancy | 3.8 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.9 | 277 | 15% PEG 3350, 0.1M SODIUM ACETATE, 0.1M ZINC ACETATE |