5Z02
Crystal structure of HIS6-tagged Mdm2 with nutlin-3a
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-12-01 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 42.500, 42.990, 54.530 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.520 - 1.350 |
R-factor | 0.2273 |
Rwork | 0.226 |
R-free | 0.25440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4j3e |
RMSD bond length | 0.024 |
RMSD bond angle | 2.314 |
Data reduction software | HKL-2000 |
Data scaling software | xia2 |
Phasing software | BUCCANEER |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 33.520 | 33.520 | 1.390 |
High resolution limit [Å] | 1.350 | 6.040 | 1.350 |
Rmerge | 0.048 | 0.033 | |
Number of reflections | 21289 | 1182 | |
<I/σ(I)> | 2.01 | ||
Completeness [%] | 99.5 | 99.6 | 99.6 |
Redundancy | 12.4 | 10.5 | 12.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.2 Ammonium acetate, 20% ammonium sulfate |