5Z02

Crystal structure of HIS6-tagged Mdm2 with nutlin-3a

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-12-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	42.500, 42.990, 54.530
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.520 - 1.350
R-factor	0.2273
Rwork	0.226
R-free	0.25440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4j3e
RMSD bond length	0.024
RMSD bond angle	2.314
Data reduction software	HKL-2000
Data scaling software	xia2
Phasing software	BUCCANEER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	33.520	33.520	1.390
High resolution limit [Å]	1.350	6.040	1.350
Rmerge	0.048	0.033
Number of reflections	21289		1182
<I/σ(I)>	2.01
Completeness [%]	99.5	99.6	99.6
Redundancy	12.4	10.5	12.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.2 Ammonium acetate, 20% ammonium sulfate