5YWT
Crystal structure of TREX1 in complex with a duplex DNA with 3' overhang
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-07-25 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.170, 80.350, 85.220 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.790 - 1.700 |
| R-factor | 0.196 |
| Rwork | 0.194 |
| R-free | 0.21900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mxm |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.871 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.077 | 0.484 |
| Number of reflections | 50099 | |
| <I/σ(I)> | 20.5 | 2.3 |
| Completeness [%] | 98.2 | 96.6 |
| Redundancy | 6.6 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.1M BICINE PH 8.5, 20%(W/V) POLYETHYLENE GLYCOL 10,000, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K |
