5YVH

Crystal structure of Karyopherin beta2 in complex with FUS(371-526)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	93
Detector technology	CCD
Collection date	2013-02-14
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97926
Spacegroup name	P 21 21 2
Unit cell lengths	129.425, 156.532, 67.822
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.355 - 3.150
R-factor	0.2155
Rwork	0.213
R-free	0.26240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4fdd
RMSD bond length	0.008
RMSD bond angle	1.160
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.200
High resolution limit [Å]	3.150	3.150
Rpim	0.051	0.533
Number of reflections	25064
<I/σ(I)>	5.4	1.34
Completeness [%]	99.6
Redundancy	5.4
CC(1/2)		0.680

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.8M Succinic acid pH7.0