5YPL
Crystal structure of NDM-1 bound to hydrolyzed imipenem representing an EP complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97845 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 69.220, 73.760, 77.430 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.280 - 1.800 |
| R-factor | 0.1705 |
| Rwork | 0.169 |
| R-free | 0.20380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4rl0 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.280 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 35229 | |
| <I/σ(I)> | 16.8 | 2.55 |
| Completeness [%] | 94.0 | 64.8 |
| Redundancy | 6.36 | 4.44 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 28% (w/v) PEG3350, 0.1M Bis-Tris, pH 5.8, 0.2M ammonium sulfate |
