5Y31
Crystal structure of human LGI1-ADAM22 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-04-15 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 105.143, 124.324, 164.663 |
| Unit cell angles | 90.00, 104.80, 90.00 |
Refinement procedure
| Resolution | 48.993 - 7.125 |
| R-factor | 0.2676 |
| Rwork | 0.265 |
| R-free | 0.31630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.819 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 7.240 |
| High resolution limit [Å] | 7.120 | 7.120 |
| Number of reflections | 6092 | |
| <I/σ(I)> | 13.3 | 4.6 |
| Completeness [%] | 97.9 | 96.3 |
| Redundancy | 7.1 | 4.6 |
| CC(1/2) | 0.578 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 10 % PEG 8000, 0.1 M zinc acetate, 0.1 M MES-Na (pH 6.0) |
