5Y2Z
Crystal structure of human LGI1 EPTP-ADAM22 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-15 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 83.579, 83.608, 293.543 |
| Unit cell angles | 86.40, 88.17, 59.95 |
Refinement procedure
| Resolution | 48.827 - 2.670 |
| R-factor | 0.246 |
| Rwork | 0.244 |
| R-free | 0.27920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3g5c |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.777 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.720 |
| High resolution limit [Å] | 2.670 | 2.660 |
| Number of reflections | 177282 | |
| <I/σ(I)> | 9.9 | |
| Completeness [%] | 90.6 | 88.4 |
| Redundancy | 3.8 | 3.7 |
| CC(1/2) | 0.702 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 10 % PEG 8000, 0.1 M zinc acetate, 0.1 M MES-Na (pH 6.0) |
