5XWD
Crystal structure of the complex of 059-152-Fv and EGFR-ECD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-02-16 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 203.428, 203.428, 113.817 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.494 - 2.894 |
| R-factor | 0.2795 |
| Rwork | 0.277 |
| R-free | 0.33090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3njp 3b2u |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.560 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.000 |
| High resolution limit [Å] | 2.894 | 2.900 |
| Number of reflections | 680489 | |
| <I/σ(I)> | 39.8 | |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 21.7 | 22.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | MES buffer (pH 6.0), zinc acetate, PEG8000 |
