5XVE
Crystal structure of human USP2 C276S mutant in complex with ubiquitin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13C1 |
| Synchrotron site | NSRRC |
| Beamline | BL13C1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-11-24 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.976 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 102.473, 54.011, 74.753 |
| Unit cell angles | 90.00, 107.79, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.240 |
| R-factor | 0.14784 |
| Rwork | 0.147 |
| R-free | 0.16403 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hd5 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.246 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.280 |
| High resolution limit [Å] | 1.240 | 1.240 |
| Rmerge | 0.034 | 0.241 |
| Number of reflections | 102971 | 14717 |
| <I/σ(I)> | 33 | |
| Completeness [%] | 98.5 | 96.9 |
| Redundancy | 3.6 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 295 | 21% PEG3350, 8% Tacsimate (pH8.0) |
