5XH7
Crystal structure of the Acidaminococcus sp. BV3L6 Cpf1 RR variant in complex with crRNA and target DNA (TCCA PAM)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-12-18 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 80.656, 133.298, 200.020 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.999 - 2.000 |
R-factor | 0.1843 |
Rwork | 0.183 |
R-free | 0.21440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5b43 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.834 |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.023 |
High resolution limit [Å] | 2.000 | 2.000 |
Rpim | 0.466 | |
Number of reflections | 145886 | |
<I/σ(I)> | 10.5 | |
Completeness [%] | 99.9 | |
Redundancy | 6.4 | |
CC(1/2) | 0.841 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 7-10% PEG 3350, 100mM sodium acetate, pH 4.5, 10% 1,6-hexanediol |