5XH7

Crystal structure of the Acidaminococcus sp. BV3L6 Cpf1 RR variant in complex with crRNA and target DNA (TCCA PAM)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003677	molecular_function	DNA binding
A	0003723	molecular_function	RNA binding
A	0004519	molecular_function	endonuclease activity
A	0004530	molecular_function	deoxyribonuclease I activity
A	0006259	biological_process	DNA metabolic process
A	0016829	molecular_function	lyase activity
A	0051607	biological_process	defense response to virus

Functional Information from PDB Data

site_id	AC1
Number of Residues	6
Details	binding site for residue NA A 1401

Chain	Residue
A	LYS757
A	HOH1575
A	HOH1752
B	A-4
B	HOH263
B	HOH265

---

site_id	AC2
Number of Residues	4
Details	binding site for residue CL A 1402

Chain	Residue
A	LYS1072
A	TYR1069
A	THR1070
A	SER1071

---

site_id	AC3
Number of Residues	7
Details	binding site for residue EDO A 1403

Chain	Residue
A	HIS977
A	VAL980
A	ASP981
A	LEU1028
A	HOH1595
B	G-3
B	A-2

---

site_id	AC4
Number of Residues	5
Details	binding site for residue EDO A 1404

Chain	Residue
A	VAL13
A	SER14
A	LYS15
A	ASP1021
B	HOH252

---

site_id	AC5
Number of Residues	6
Details	binding site for residue EDO A 1405

Chain	Residue
A	THR2
A	GLN3
A	GLY6
A	PHE7
A	ARG891
A	HOH1644

---

site_id	AC6
Number of Residues	6
Details	binding site for residue EDO A 1406

Chain	Residue
A	PHE1079
A	VAL1080
A	MET1119
A	LEU1129
A	GLY1131
A	GLN1303

---

site_id	AC7
Number of Residues	4
Details	binding site for residue EDO A 1407

Chain	Residue
A	LYS559
A	ASN560
A	GLY561
A	ASP591

---

site_id	AC8
Number of Residues	4
Details	binding site for residue EDO A 1408

Chain	Residue
A	LEU95
A	ILE96
A	GLN99
A	HOH1891

---

site_id	AC9
Number of Residues	3
Details	binding site for residue EDO A 1409

Chain	Residue
A	ASP591
A	PHE769
A	HOH1506

---

site_id	AD1
Number of Residues	7
Details	binding site for residue EDO A 1410

Chain	Residue
A	LYS530
A	ARG790
A	PHE870
A	HIS872
A	HOH1682
B	U-15
B	U-16

---

site_id	AD2
Number of Residues	7
Details	binding site for residue EDO A 1411

Chain	Residue
A	GLY171
A	GLU174
A	ASN175
A	HOH1822
B	A3
C	DC-1
C	DT-2

---

site_id	AD3
Number of Residues	5
Details	binding site for residue EDO A 1412

Chain	Residue
A	PRO23
A	ASP867
A	PHE869
A	HOH1708
A	HOH1817

---

site_id	AD4
Number of Residues	3
Details	binding site for residue EDO A 1413

Chain	Residue
A	TYR687
A	LYS689
C	DG3

---

site_id	AD5
Number of Residues	7
Details	binding site for residue EDO A 1414

Chain	Residue
A	GLU581
A	VAL1247
A	PHE1249
A	ASN1255
A	TRP1258
A	HOH1781
A	HOH1889

---

site_id	AD6
Number of Residues	1
Details	binding site for residue EDO A 1415

Chain	Residue
A	GLN24

---

site_id	AD7
Number of Residues	6
Details	binding site for residue NA B 101

Chain	Residue
B	U10
B	G11
B	HOH251
B	HOH294
B	HOH309
C	HOH214

---

site_id	AD8
Number of Residues	5
Details	binding site for residue NA B 102

Chain	Residue
B	A2
B	HOH202
B	HOH212
B	HOH293
B	HOH303

---

site_id	AD9
Number of Residues	6
Details	binding site for residue NA B 103

Chain	Residue
B	U16
B	G17
B	HOH215
B	HOH260
B	HOH297
B	HOH308

---

site_id	AE1
Number of Residues	4
Details	binding site for residue EDO B 104

Chain	Residue
A	HIS755
B	C-9
B	U-8
B	U-5

---

site_id	AE2
Number of Residues	7
Details	binding site for residue EDO B 105

Chain	Residue
B	G0
B	G1
B	A2
B	HOH231
A	THR16
A	GLN784
A	GLU786

---

site_id	AE3
Number of Residues	3
Details	binding site for residue EDO B 106

Chain	Residue
A	TYR823
B	U-10
B	A-19

---

site_id	AE4
Number of Residues	6
Details	binding site for residue NA C 101

Chain	Residue
B	HOH264
B	HOH307
C	DG-14
C	HOH242
C	HOH247
C	HOH261

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	8
Details	DNA_BIND: PAM-binding on target DNA => ECO:0000269|PubMed:27114038, ECO:0000269|PubMed:27444870

Chain	Residue	Details
A	PRO599-ARG607

---

site_id	SWS_FT_FI2
Number of Residues	22
Details	DNA_BIND: Target DNA => ECO:0000269|PubMed:27114038, ECO:0000269|PubMed:27444870

Chain	Residue	Details
A	LYS780-GLY783
A	ARG951-LYS968
A	SER1051-ALA1053

---

site_id	SWS_FT_FI3
Number of Residues	3
Details	ACT_SITE: For pre-crRNA processing => ECO:0000250|UniProtKB:A0Q7Q2

Chain	Residue	Details
A	HIS800
A	LYS809
A	LYS860

---

site_id	SWS_FT_FI4
Number of Residues	3
Details	ACT_SITE: For DNase activity of RuvC domain => ECO:0000269|PubMed:27114038

Chain	Residue	Details
A	ASP908
A	GLU993
A	ASP1263

---

site_id	SWS_FT_FI5
Number of Residues	1
Details	ACT_SITE: For DNase activity of nuclease domain => ECO:0000269|PubMed:27114038

Chain	Residue	Details
A	ARG1226

---

site_id	SWS_FT_FI6
Number of Residues	4
Details	BINDING: BINDING => ECO:0000269|PubMed:27114038, ECO:0000269|PubMed:27444870

Chain	Residue	Details
A	TYR47
A	ASN175
A	LYS307
A	LYS752

---

site_id	SWS_FT_FI7
Number of Residues	1
Details	BINDING: BINDING => ECO:0000269|PubMed:27114038

Chain	Residue	Details
A	MET806

---

site_id	SWS_FT_FI8
Number of Residues	3
Details	SITE: Binds crRNA => ECO:0000269|PubMed:27114038, ECO:0000269|PubMed:27444870

Chain	Residue	Details
A	ARG18
A	ARG192
A	HIS872

---

site_id	SWS_FT_FI9
Number of Residues	2
Details	SITE: Binds PAM on target DNA => ECO:0000269|PubMed:27114038, ECO:0000269|PubMed:27444870

Chain	Residue	Details
A	THR167
A	LYS548

---

site_id	SWS_FT_FI10
Number of Residues	1
Details	SITE: Binds crRNA-target DNA heteroduplex => ECO:0000269|PubMed:27114038, ECO:0000269|PubMed:27444870

Chain	Residue	Details
A	TRP382

---

site_id	SWS_FT_FI11
Number of Residues	1
Details	SITE: Binds sequence-specific recognition of both target and non-target strand bases in PAM => ECO:0000269|PubMed:27114038, ECO:0000269|PubMed:27444870

Chain	Residue	Details
A	ARG607

---

site_id	SWS_FT_FI12
Number of Residues	1
Details	SITE: Binds target DNA => ECO:0000269|PubMed:27114038

Chain	Residue	Details
A	GLN1014

---