5XFV
Crystal structures of FMN-bound form of dihydroorotate dehydrogenase from Trypanosoma brucei
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2012-11-22 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 139.914, 146.979, 66.491 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.980 - 1.790 |
| R-factor | 0.16753 |
| Rwork | 0.166 |
| R-free | 0.19762 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b4g |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.181 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.830 |
| High resolution limit [Å] | 1.790 | 1.800 |
| Rmerge | 0.077 | |
| Number of reflections | 128274 | |
| <I/σ(I)> | 7.1 | 7.1 |
| Completeness [%] | 99.0 | 98.5 |
| Redundancy | 11.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 0.1M Sodium Citrate buffer, 1.5-2.5 M Sodium Malonate |
