5XFV

Crystal structures of FMN-bound form of dihydroorotate dehydrogenase from Trypanosoma brucei

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Dihydroorotate dehydrogenase (fumarate)	polymer	334	36463.9	4	UniProt (Q57U83) Pfam (PF01180)	Trypanosoma brucei brucei strain 927/4 GUTat10.1	DHOdehase,Dihydroorotate oxidase
2	E, I, M, R (A, B, C, D)	FLAVIN MONONUCLEOTIDE	non-polymer		456.3	4	Chemie (FMN) PubChem (710) PubChem (23585816) PubChem (8965) PubChem (51435500) PubChem (51435503) PubChem (643976) PubChem (5702760) PubChem (7048779) PubChem (7048781) PubChem (15559251)
3	F, G, H, J, K... (A, B, C, D)	MALONATE ION	non-polymer		102.0	11	Chemie (MLI) PubChem (9084)
4	T, U, V, W (A, B, C, D)	water	water		18.0	752	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 313 (UniProt: Q57U83)

PDB	External Database	Details
Met -20	-	expression tag
Ala -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
Ser -12	-	expression tag
Ala -11	-	expression tag
Ala -10	-	expression tag
Leu -9	-	expression tag
Glu -8	-	expression tag
Val -7	-	expression tag
Leu -6	-	expression tag
Phe -5	-	expression tag
Gln -4	-	expression tag
Gly -3	-	expression tag
Pro -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Val 115	Ala 115	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	145855.6
Non-Polymers*	Number of molecules	15
	Total formula weight	2947.9
All*	Total formula weight	148803.5

*Water molecules are not included.