5X07
Crystal structure of FOXA2 DNA binding domain bound to a full consensus DNA site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-06-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9789 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 195.126, 71.932, 72.155 |
| Unit cell angles | 90.00, 103.14, 90.00 |
Refinement procedure
| Resolution | 33.637 - 2.796 |
| R-factor | 0.2431 |
| Rwork | 0.240 |
| R-free | 0.27850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.108 |
| Data reduction software | HKL-3000 |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 33.640 |
| High resolution limit [Å] | 2.796 |
| Number of reflections | 178752 |
| <I/σ(I)> | 19.47 |
| Completeness [%] | 96.8 |
| Redundancy | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 80mM Mg (OAc)2, 50mM MES buffer, 16%-20% PEG4K, pH 6.5 |
