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5X07

Crystal structure of FOXA2 DNA binding domain bound to a full consensus DNA site

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSSRF BEAMLINE BL17U
Synchrotron siteSSRF
BeamlineBL17U
Temperature [K]100
Detector technologyCCD
Collection date2016-06-01
DetectorADSC QUANTUM 315r
Wavelength(s)0.9789
Spacegroup nameC 1 2 1
Unit cell lengths195.126, 71.932, 72.155
Unit cell angles90.00, 103.14, 90.00
Refinement procedure
Resolution33.637 - 2.796
R-factor0.2431
Rwork0.240
R-free0.27850
Structure solution methodMOLECULAR REPLACEMENT
RMSD bond length0.005
RMSD bond angle1.108
Data reduction softwareHKL-3000
Refinement softwarePHENIX (1.9_1692)
Data quality characteristics
 Overall
Low resolution limit [Å]33.640
High resolution limit [Å]2.796
Number of reflections178752
<I/σ(I)>19.47
Completeness [%]96.8
Redundancy7.5
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP27780mM Mg (OAc)2, 50mM MES buffer, 16%-20% PEG4K, pH 6.5

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