5WYN
HtrA2 Pathogenic Mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | RRCAT INDUS-2 BEAMLINE PX-BL21 |
| Synchrotron site | RRCAT INDUS-2 |
| Beamline | PX-BL21 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-03-10 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9795 |
| Spacegroup name | H 3 |
| Unit cell lengths | 85.646, 85.646, 126.306 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.823 - 2.050 |
| R-factor | 0.1568 |
| Rwork | 0.153 |
| R-free | 0.18540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lcy |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.125 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.080 | |
| High resolution limit [Å] | 2.050 | |
| Number of reflections | 21668 | |
| <I/σ(I)> | 16.4 | 1.23 |
| Completeness [%] | 99.8 | |
| Redundancy | 4.2 | |
| CC(1/2) | 0.391 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | Sodium phosphate monobasic monohydrate Potassium phosphate monobasic Sodium chloride |
