5WLB

KRas G12V, bound to GppNHp and miniprotein 225-15a/b

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2014-11-08
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97918
Spacegroup name	P 1 21 1
Unit cell lengths	38.145, 86.843, 71.038
Unit cell angles	90.00, 103.57, 90.00

Refinement procedure

Resolution	69.100 - 1.720
R-factor	0.1942
Rwork	0.192
R-free	0.23320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3gft
RMSD bond length	0.010
RMSD bond angle	1.191
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	69.100	1.810
High resolution limit [Å]	1.720	1.720
Rmerge	0.068
Number of reflections	47613
<I/σ(I)>	13.9
Completeness [%]	99.3
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.2 M AMMONIUM SULFATE, 26% PEG 3350, 0.1M HEPES PH 7.5