5W9A
The structure of the Trim5alpha Bbox- coiled coil in complex LC3B
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2016-11-08 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 1.25 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 72.014, 115.319, 174.164 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.910 | 40.910 | 2.969 |
High resolution limit [Å] | 2.740 | 8.961 | 2.740 |
Rmerge | 0.115 | 0.046 | 1.820 |
Rmeas | 0.120 | 0.048 | 1.892 |
Rpim | 0.034 | 0.014 | 0.514 |
Total number of observations | 298683 | 12528 | 16041 |
Number of reflections | 24308 | 1214 | 1215 |
<I/σ(I)> | 13 | 40.1 | 1.5 |
Completeness [%] | 92.9 | 98.2 | 61.7 |
Redundancy | 12.3 | 10.3 | 13.2 |
CC(1/2) | 0.999 | 0.998 | 0.367 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | 0.2 M NH4Cl, 0.1 M Tris pH8, 20% PEG 6,000 |