5W59
Crystal structure of a monomeric human FGF9 in complex with the ectodomain of human FGFR1c
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4C |
Synchrotron site | NSLS |
Beamline | X4C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-03-14 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.97900 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 42.159, 86.793, 136.857 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.527 - 2.498 |
R-factor | 0.176 |
Rwork | 0.171 |
R-free | 0.22110 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ojv |
RMSD bond length | 0.008 |
RMSD bond angle | 0.956 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.540 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 18063 | 867 |
<I/σ(I)> | 29.8 | 4 |
Completeness [%] | 99.4 | 97.6 |
Redundancy | 6.3 | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 100 mM Tris, pH 8.0, 8% w/v PEG20000, 0.3 M sodium chloride, 40 mM L-proline |