5VQF

Crystal Structure of pro-TGF-beta 1

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2009-11-12
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97934, 0.97932, 0.97956, 0.95667
Spacegroup name	P 1 21 1
Unit cell lengths	54.660, 126.920, 137.920
Unit cell angles	90.00, 96.70, 90.00

Refinement procedure

Resolution	37.142 - 2.900
R-factor	0.2406
Rwork	0.239
R-free	0.27980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3rjr
RMSD bond length	0.005
RMSD bond angle	0.849
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SOLVE
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.980
High resolution limit [Å]	2.900	2.900
Rmerge	0.052	2.740
Number of reflections	39918	2994
<I/σ(I)>	15.3	0.55
Completeness [%]	96.1	98
Redundancy	4.3	4.3
CC(1/2)	0.999	0.330

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	7% PEG 3350, 17% isopropanol, 0.1m Na citrate pH 5.6