Loading
PDBj
MenuPDBj@FacebookPDBj@TwitterPDBj@YouTubewwPDB FoundationwwPDB
RCSB PDBPDBeBMRBAdv. SearchSearch help

5VK0

Crystal structure of human MDM2 in complex with a 12-mer lysine-cysteine side chain dithiocarbamate stapled peptide inhibitor PMI

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSSRL BEAMLINE BL12-2
Synchrotron siteSSRL
BeamlineBL12-2
Temperature [K]100
Detector technologyPIXEL
Collection date2017-01-15
DetectorDECTRIS PILATUS3 S 6M
Wavelength(s)0.97946
Spacegroup nameC 2 2 21
Unit cell lengths90.838, 157.470, 196.650
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution41.700 - 1.800
R-factor0.20046
Rwork0.198
R-free0.24656
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)3eqs
RMSD bond length0.020
RMSD bond angle2.495
Data reduction softwareHKL-2000
Data scaling softwareHKL-2000
Phasing softwarePHASER
Refinement softwareREFMAC (5.8.0158)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0001.830
High resolution limit [Å]1.8001.800
Rmerge0.0760.299
Number of reflections123372
<I/σ(I)>23.23
Completeness [%]95.597.9
Redundancy3.43.4
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP6.52941340 mM ammonium sulfate, 6.7% glycerol, 50 mM magnesium sulfate, 100 mM imidazole pH 6.5

225158

PDB entries from 2024-09-18

PDB statisticsPDBj update infoContact PDBjnumon