5VAJ
BhRNase H - amide-RNA/DNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2016-03-10 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 0.97856 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 137.180, 37.513, 93.863 |
| Unit cell angles | 90.00, 121.65, 90.00 |
Refinement procedure
| Resolution | 79.900 - 1.950 |
| R-factor | 0.16813 |
| Rwork | 0.166 |
| R-free | 0.21694 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zbi |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.380 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.090 | 0.460 |
| Number of reflections | 30154 | 2958 |
| <I/σ(I)> | 18.47 | |
| Completeness [%] | 99.9 | 99.6 |
| Redundancy | 5.1 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 50 mM sodium cacodylate (pH 6.5) and 0.7 M sodium acetate. |
