5V87

Crystal structure of LARP1-unique domain DM15 bound to m7GpppC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E DW
Temperature [K]	90
Detector technology	IMAGE PLATE
Collection date	2017-01-04
Detector	RIGAKU RAXIS HTC
Wavelength(s)	1.54
Spacegroup name	P 1 21 1
Unit cell lengths	47.959, 59.962, 60.321
Unit cell angles	90.00, 100.65, 90.00

Refinement procedure

Resolution	24.986 - 1.692
R-factor	0.1828
Rwork	0.181
R-free	0.20350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5c0v
RMSD bond length	0.008
RMSD bond angle	0.884
Data scaling software	HKL-2000
Phasing software	PHENIX (1.11.1_2575)
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	1.690
Number of reflections	34328
<I/σ(I)>	19.85
Completeness [%]	92.2
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.05 M Sodium cacodylate trihydrate pH 6.5, 30% w/v Polyethylene glycol 4,000 (#25 Index-116)