5V39
Crystal structure of human vitamin D receptor ligand binding domain in complex with a VDRM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-01 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | .97931 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.000, 52.670, 105.880 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.310 - 2.200 |
| R-factor | 0.172 |
| Rwork | 0.167 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.000 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.310 |
| High resolution limit [Å] | 2.200 |
| Number of reflections | 13006 |
| <I/σ(I)> | 8.56 |
| Completeness [%] | 99.9 |
| Redundancy | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 298 | 100mM Tris HCl pH 8.5, 25% PEG 3350 and 200mM Ammonium Sulfate |
