5UVV
BRD4 Bromodomain 2 with A-1457066
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-10-13 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 33.465, 85.769, 40.551 |
Unit cell angles | 90.00, 111.40, 90.00 |
Refinement procedure
Resolution | 17.480 - 1.990 |
R-factor | 0.247 |
Rwork | 0.245 |
R-free | 0.30100 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.830 |
Data reduction software | XDS (2.11.7) |
Data scaling software | SCALA |
Refinement software | BUSTER (2.11.7) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 34.550 |
High resolution limit [Å] | 1.990 |
Rmerge | 0.049 |
Number of reflections | 14372 |
<I/σ(I)> | 13.4 |
Completeness [%] | 98.5 |
Redundancy | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 277 | Protein Buffer : 10 mM HEPES PH 7.5 100 mM NaCl 5mM DTT Crystallization : 15 % (v/v) Ethanol Tris PH 7.0 |