5UN5
Frizzled-8 complex with designed surrogate Wnt agonist, crystal form 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-09-22 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 41.200, 77.510, 81.430 |
Unit cell angles | 90.00, 92.76, 90.00 |
Refinement procedure
Resolution | 41.152 - 2.994 |
R-factor | 0.2278 |
Rwork | 0.225 |
R-free | 0.24990 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4f0a |
RMSD bond length | 0.002 |
RMSD bond angle | 0.533 |
Data reduction software | XDS (March 30, 2013) |
Data scaling software | XSCALE (2015-08-21) |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.152 | 3.050 |
High resolution limit [Å] | 2.980 | 2.980 |
Number of reflections | 10346 | 679 |
<I/σ(I)> | 11.5 | 1.08 |
Completeness [%] | 99.1 | 88.8 |
Redundancy | 4.1 | 3.6 |
CC(1/2) | 0.997 | 0.577 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 42-49% PEG 400, 0.1 M Tris pH 7.8-8.2, 0.2 M NaCl |