5UKM
bovine GRK2 in complex with human Gbetagamma subunits and CCG258208 (14as)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-07-16 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.97857 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 189.012, 74.150, 123.175 |
Unit cell angles | 90.00, 115.46, 90.00 |
Refinement procedure
Resolution | 30.000 - 3.030 |
R-factor | 0.1997 |
Rwork | 0.197 |
R-free | 0.25160 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3v5w |
RMSD bond length | 0.012 |
RMSD bond angle | 1.563 |
Data scaling software | XSCALE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 3.210 |
High resolution limit [Å] | 3.030 | 8.940 | 3.030 |
Rmerge | 0.119 | 0.038 | 0.932 |
Rmeas | 0.129 | 0.041 | 1.007 |
Total number of observations | 207868 | ||
Number of reflections | 29942 | 1226 | 4657 |
<I/σ(I)> | 12.84 | 41.18 | 1.98 |
Completeness [%] | 98.5 | 97.8 | 96 |
Redundancy | 6.942 | 6.748 | 7.055 |
CC(1/2) | 0.998 | 0.999 | 0.894 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 100 mM MES, 0.2 M NaCl, 8-10% PEG3350 |