5UKM

bovine GRK2 in complex with human Gbetagamma subunits and CCG258208 (14as)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Beta-adrenergic receptor kinase 1	polymer	695	80578.8	1	UniProt (P21146) Pfam (PF00615) Pfam (PF00069) Pfam (PF00169)	Bos taurus (Bovine)	Beta-ARK-1,G-protein-coupled receptor kinase 2
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	350	38534.1	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (Human)	Transducin beta chain 1
3	C (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (Human)	G gamma-I
4	D (A)	5-[(3S,4R)-3-{[(2H-1,3-benzodioxol-5-yl)oxy]methyl}piperidin-4-yl]-2-fluoro-N-[(1H-pyrazol-5-yl)methyl]benzamide	non-polymer		452.5	1	Chemie (T0E)
5	E (A)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
6	F (G)	O-METHYLCYSTEINE	non-polymer		135.2	1	Chemie (CMT)
7	G, H (A, B)	water	water		18.0	23	Chemie (HOH)

Sequence modifications

A: 1 - 689 (UniProt: P21146)

PDB	External Database	Details
Ala 670	Ser 670	engineered mutation
His 690	-	expression tag
His 691	-	expression tag
His 692	-	expression tag
His 693	-	expression tag
His 694	-	expression tag
His 695	-	expression tag

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	126974.0
Non-Polymers*	Number of molecules	3
	Total formula weight	612.0
All*	Total formula weight	127586.0

*Water molecules are not included.