5UHK
Crystal structure of the core catalytic domain of Human O-GlcNAcase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-03-30 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 96.800, 96.800, 256.960 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.240 - 2.970 |
| R-factor | 0.197 |
| Rwork | 0.194 |
| R-free | 0.25100 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.120 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.6) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 128.000 | 2.980 |
| High resolution limit [Å] | 2.970 | 2.970 |
| Rmerge | 0.077 | 1.055 |
| Number of reflections | 24942 | |
| <I/σ(I)> | 22.3 | 2.2 |
| Completeness [%] | 95.9 | 100 |
| Redundancy | 12.8 | 12.6 |
| CC(1/2) | 0.999 | 0.927 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 14-17% PEG 3350, 0.2 M Mg Formate |
