5UAN
Crystal structure of multi-domain RAR-beta-RXR-alpha heterodimer on DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-08-03 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.470, 77.403, 112.113 |
| Unit cell angles | 90.00, 90.37, 90.00 |
Refinement procedure
| Resolution | 47.405 - 3.508 |
| R-factor | 0.2225 |
| Rwork | 0.221 |
| R-free | 0.27030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dsz 1xdk |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.546 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.560 |
| High resolution limit [Å] | 3.500 | 9.480 | 3.500 |
| Rmerge | 0.110 | 0.047 | 0.431 |
| Rmeas | 0.125 | ||
| Rpim | 0.057 | ||
| Total number of observations | 38824 | ||
| Number of reflections | 9550 | ||
| <I/σ(I)> | 7.1 | ||
| Completeness [%] | 84.0 | 99 | 52.2 |
| Redundancy | 4.1 | 5.4 | 2.5 |
| CC(1/2) | 0.974 | 0.780 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | MES, PEG300 |
